Bruno Botta
Dipartimento di Chimica e Tecnologie del Farmaco
Sapienza Università di Roma

Piazzale A. Moro 5

00185 Roma

Ex-Palazzina Alloggi, 2° piano, stanza n° 23

Tel.: 0649912781 (interno: 2-2781)

Fax: 0649912780

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Professor Bruno Botta

Bruno Botta is full professor and he has been nominated Deputy Rector for the Internationalization  in November 2014. He is Head of the Dipartimento di Chimica e Tecnologie del Farmaco at Sapienza University of Rome since 2011. His interest during the years has been focused on the structural elucidation and synthesis of biologically active compounds derived from living plants. Since 20 years, he has been working on the field of plant tissue cultures in combination with chemistry directed toward the understanding of biosynthetic pathways of the compounds under investigation. During the years, some plant enzymes (such as those belonging to the peroxidases family) derived from the above-mentioned cell cultures have been isolated and purified and some kinetic studies have been performed too. During the last 15 years, Prof. Botta focused his attention also on both the synthesis and host-guest studies of artificial receptors of the resorcarenes family.

During the last 7 years  he focused his attention on the Hedgehog (Hh) signalling pathway which plays a pivotal role in the initiation, proliferation, invasion and metastasis of various cancers (inter aliamedulloblastoma).

He is author of 130 publications, including 5 patents (3 national and 2 international – USA and Europe), and, besides that, he is author and co-author of 10 books. For the Italian publishing house Edi-Ermes he edited 2 text books of Organic Chemistry.

Bruno Botta was born in 1954 and got his Laurea in Chemistry in 1977 at the University of Rome "La Sapienza". During the years 1984-1985, he was appointed as postdoctoral associate at the Department of Chemistry of the University of British Columbia (UBC, Vancouver, Canada) working on the synthesis and biosynthesis of clinically important anticancer alkaloids of the vinblastine-vincristine family. From 1981 to 1992 he was appointed as assistant professor (ricercatore) at the Catholic University of Rome. From 1992 to 2001 he was appointed as associate professor of organic chemistry at the University of Rome "La Sapienza" (Faculty of Pharmacy) and became full professor at the same University in 2001. Since 2011, he is Head of the Dipartimento di Chimica e Tecnologie del Farmaco of the University of Rome "La Sapienza".


He was invited to give lectures in the following universities: Michigan State University (USA), Wofford College Spartanburg (SC, USA), The Scripps Research Institute, La Jolla (CA, USA), University of British Columbia (Vancouver, Canada), Universidad de Montevideo (Uruguay), Stanford University (CA, USA), Universidade Federal de Pernambuco (Brazil), Toho University, Chiba, Tokyo (Japan). 

He is author of 130 publications, including 5 patents (3 national and 2 international – USA and Europe), and, beside that, he is author and co-author of 10 books. For the Italian publishing house Edi-Ermes he edited 2 text books of Organic Chemistry.

 

 

 

Selected Publications (2004-2015)

 

 

1.    Infante, P., Alfonsi, R., Botta, B., Mori, M., Di Marcotullio, L.. Targeting Gli factors to inhibit the Hedgehog pathway. Trends in Pharmacological Sciences, In press.

2.    Infante, P., Mori, M., Alfonsi, R., Ghirga, F., Aiello, F., Toscano S., Ingallina C., Siler M., Cucchi D., Po A., Miele E., D’Amico D., Canettieri, G.,  De Smaele, E.,  Ferretti, E.,  Screpanti, I., Uccello Barretta, G., Botta, M., Botta, B., Gulino, A., and  Di Marcotullio, L.. Gli1/DNA interaction is a druggable target for Hedgehogdependent tumors. EMBO Journal (2015) 34(2), 200-217.

3.    Uccello-Barretta, G., Schurig, V., Balzano, F., Vanni, L., Aiello, F., Mori, M., Ghirga, F.. Synergistic Effects of Trace Amounts of Water in the Enantiodiscrimination Processes by Lipodex E: A Spectroscopic and Computational Investigation. Chirality (2015)  27(2), 95-103.

4.    Uccello-Barretta, G., Balzano, F., Aiello, F., Vanni, L., Mori, M., Menta, S., Calcaterra, A., Botta, B. Hydrolytic inhibition of α-chymotrypsin by 2,8,14,20-tetrakis(d-leucyl-d-valinamido) resorc[4]arenecarboxylic acid: a spectroscopic NMR and computational combined approach. Org. Biomol. Chem.(2015), 13(3), 916-924.   

5.    Uccello-Barretta, G., Balzano, F., Aiello, F., Vanni, L., Mori, M., Menta, S., Calcaterra, A., Botta, B. Hydrolytic inhibition of α-chymotrypsin by 2,8,14,20-tetrakis(d-leucyl-d-valinamido) resorc[4]arenecarboxylic acid: a spectroscopic NMR and computational combined approach (2014) Org. Biomol. Chem. [DOI: 10.1039/C4OB01936A]     

6.    Ghirga, F., Quaglio, D., Iovine, V., Botta, B., Pierini, M., Mannina, L., Sobolev, A. P., Ugozzoli, F., D' Acquarica, I. Synthesis of a Double-Spanned-Resorc[4]arene via Ring-Closing Metathesis and Calculation of Aggregation Propensity (2014) J. Org. Chem. 79 (22), 11051–11060.

7.    D'Acquarica, I., Calcaterra, A., Sacco, F., Balzano, F., Aiello, F., Tafi, A., Pesci, N., Uccello-Barretta, G., Botta, B. Stereochemical preference of 2'-deoxycytidine for chiral bis(diamido)-bridged basket resorcin[4]arenes (2013) Chirality, 25 (12), pp. 840-851. 

8.    Cerreto, A., Corrente, F., Botta, B., Pacelli, S., Paolicelli, P., Mannina, L., Casadei, M.A. NMR Characterization of Carboxymethyl Scleroglucan(2013) International Journal of Polymer Analysis and Characterization, 18 (8), pp. 587-595. 

9.    Ghirga, F., D'Acquarica, I., Delle Monache, G., Toscano, S., Mannina, L., Sobolev, A.P., Ugozzoli, F., Crocco, D., Antiochia, R., Botta, B. Undecenyl resorc[4]arene in the chair conformation as preorganized synthon for olefin metathesis(2013) RSC Advances, 3 (38), pp. 17567-17576.

10.  Mascarello, A., Mori, M., Chiaradia-Delatorre, L.D., Menegatti, A.C.O., de Monache, F., Ferrari, F., Yunes, R.A., Nunes, R.J., Terenzi, H., Botta, B., Botta, M. Discovery of Mycobacterium tuberculosis Protein Tyrosine Phosphatase B (PtpB) Inhibitors from Natural Products (2013) PLoS ONE, 8 (10), art. no. e77081.

11.  Rondino, F., Ciavardini, A., Satta, M., Paladini, A., Fraschetti, C., Filippi, A., Botta, B., Calcaterra, A., Speranza, M., Giardini, A., Piccirillo, S. Ultraviolet and infrared spectroscopy of neutral and ionic non-covalent diastereomeric complexes in the gas phase(2013) Rendiconti Lincei, 24 (3), pp. 259-267. 

12.  Ghirga, F., D'Acquarica, I., Delle Monache, G., Mannina, L., Molinaro, C., Nevola, L., Sobolev, A.P., Pierini, M., Botta, B. Reaction of nitrosonium cation with resorc[4]arenes activated by supramolecular control: Covalent bond formation(2013) Journal of Organic Chemistry, 78 (14), pp. 6935-6946. 

13.  Friggeri, L., Scipione, L., Costi, R., Kaiser, M., Moraca, F., Zamperini, C., Botta, B., Di Santo, R., De Vita, D., Brun, R., Tortorella, S. New promising compounds with in vitro nanomolar activity against Trypanosoma cruzi(2013) ACS Medicinal Chemistry Letters, 4 (6), pp. 538-541. 

14.  Filippi, A., Fraschetti, C., Piccirillo, S., Rondino, F., Botta, B., D'Acquarica, I., Calcaterra, A., Speranza, M. Chirality effects on the IRMPD spectra of basket resorcinarene/nucleoside complexes(2012) Chemistry - A European Journal, 18 (27), pp. 8320-8328. 

15.  Menendez, P., D'Acquarica, I., Delle Monache, G., Ghirga, F., Calcaterra, A., Barba, M., Macone, A., Boffi, A., Bonamore, A., Botta, B. Production of Bioactives Compounds: The Importance of Pictet-Spengler Reaction in the XXI Century(2012) Plant Bioactives and Drug Discovery: Principles, Practice, and Perspectives, pp. 453-487.  

16.  D’Acquarica, I., Cerreto, A., Delle Monache, G., Subrizi, F., Boffi, A., Tafi, A., Forli, S., Botta, B. N-linked peptidoresorc[4]arene-based receptors as noncompetitive inhibitors for α-chymotrypsin(2011) Journal of Organic Chemistry, 76 (11), pp. 4396-4407. 

17.  Macone, A., Fontana, M., Barba, M., Botta, B., Nardini, M., Ghirga, F., Calcaterra, A., Pecci, L., Matarese, R.M. Antioxidant properties of aminoethylcysteine ketimine decarboxylated dimer: A review(2011) International Journal of Molecular Sciences, 12 (5), pp. 3072-3084. 

18.  Botta, B., Fraschetti, C., D'Acquarica, I., Sacco, F., Mattay, J., Letzel, M.C., Speranza, M. Unprecedented gas-phase chiroselective logic gates(2011) Organic and Biomolecular Chemistry, 9 (6), pp. 1717-1719. 

19.  Bonamore, A., Rovardi, I., Gasparrini, F., Baiocco, P., Barba, M., Molinaro, C., Botta, B., Boffi, A., MacOne, A. An enzymatic, stereoselective synthesis of (S)-norcoclaurine(2010) Green Chemistry, 12 (9), pp. 1623-1627. 

20.  Basile, A., Botta, B., Bruno, M., Rigano, D., Sorbo, S., Conte, B., Rosselli, S., Senatore, F. Effects of air pollution on production of essential oil in Feijoa Sellowiana Berg. grown in the 'Italian triangle of death'(2010) International Journal of Environment and Health, 4 (2-3), pp. 250-259. 

21.  Bonamore, A., Barba, M., Botta, B., Boffi, A., Macone, A. Norcoclaurine synthase: Mechanism of an enantioselective Pictet-Spengler catalyzing enzyme(2010) Molecules, 15 (4), pp. 2070-2078. 

22.  Speranza, M., D'Acquarica, I., Fraschetti, C., Botta, B., Tafi, A., Bellucci, L., Zappia, G. Diastereoselective gas-phase ion/molecule reactions of ethanolamine neurotransmitter/amido[4]resorcinarene adducts (2010) International Journal of Mass Spectrometry, 291 (1-2), pp. 84-89.

23.  Botta, B., Fraschetti, C., D'Acquarica, I., Speranza, M., Novara, F.R., Mattay, J., Letzel, M.C. Gas-Phase enantioselectivity of chiral N-Linked peptidoresorc[4]arene isomers toward dipeptides (2009) Journal of Physical Chemistry A, 113 (52), pp. 14625-14629. 

24.  Torge, R., Comandini, A., Catacchio, B., Bonamore, A., Botta, B., Boffi, A.  Peroxidase-like activity of Thermobifida fusca hemoglobin: The oxidation of dibenzylbutanolide (2009) Journal of Molecular Catalysis B: Enzymatic, 61 (3-4), pp. 303-308. 

25.  Botta, B., Menendez, P., Zappia, G., De Lima, R.A., Torge, R., Delle Monache, G. Prenylated isoflavonoids: Botanical distribution, structures, biological activities and biotechnological studies. An update (1995-2006) (2009) Current Medicinal Chemistry, 16 (26), pp. 3414-3468. 

26.  Zappia, G., Menendez, M.P., De Andrade Lima, C.S., Botta, B. A p-quinol isoflavan and two new isoflavanones from Desmodium canum (2009) Natural Product Research, 23 (7), pp. 665-671. 

27.  Botta, B., Fraschetti, C., Novara, F.R., Tafi, A., Sacco, F., Mannina, L., Sobolev, A.P., Mattay, J., Letzel, M.C., Speranza, M. Interactions of vinca alkaloid subunits with chiral amido[4]resorcinarenes: A dynamic, kinetic, and spectroscopic study (2009) Organic and Biomolecular Chemistry, 7 (9), pp. 1798-1806. 

28.  Speranza, M., Gasparrini, F., Botta, B., Villani, C., Subissati, D., Fraschetti, C., Subrizi, F.  Gas-phase enantioselective reactions in noncovalent ion-molecule complexes (2009) Chirality, 21 (1), pp. 69-86. 

29.  Ilari, A., Franceschini, S., Bonamore, A., Arenghi, F., Botta, B., Macone, A., Pasquo, A., Bellucci, L., Boffi, A. Structural basis of enzymatic (S)-norcoclaurine biosynthesis (2009) Journal of Biological Chemistry, 284 (2), pp. 897-904. 

30.  Botta, B., Pierini, M., Delle Monache, G., Subissati, D., Subrizi, F., Zappia, G. Synthesis of amino and ammonium resorcin[4]arenes as potential receptors (2008) Synthesis, (13), pp. 2110-2116. 

31.  Botta, B., Tafi, A., Caporuscio, F., Botta, M., Nevola, L., D'Acquarica, I., Frascherti, C., Speranza, M. Modelling amphetamine/receptor interactions: A gas-phase study of complexes formed between amphetamine and some chiral amido[4] resorcinarenes (2008) Chemistry - A European Journal, 14 (12), pp. 3585-3595. 

32.  Poce, G., Zappia, G., Porretta, G.C., Botta, B., Biava, M. New oxazolidinone derivatives as antibacterial agents with improved activity (2008) Expert Opinion on Therapeutic Patents, 18 (2), pp. 97-121. 

33.  Botta, B., D'Acquarica, I., Nevola, L., Sacco, F., Valbuena Lopez, Z., Zappia, G., Fraschetti, C., Speranza, M., Tafi, A., Caporuscio, F., Letzel, M.C., Mattay, J. Bis(diamido)-bridged basket resorcin[4]arenes as enantioselective receptors for amino acids and amines (2007) European Journal of Organic Chemistry, (36), pp. 5995-6002.

34.  Zappia, G., Gacs-Baitz, E., Delle Monache, G., Misiti, D., Nevola, L., Botta, B. Oxazolidin-2-one ring, a popular framework in synthetic organic chemistry: Part 1. The construction of the oxazolidin-2-one ring (2007) Current Organic Synthesis, 4 (1), pp. 81-135.

35.  Zappia, G., Cancelliere, G., Gacs-Baitz, E., Delle Monache, G., Misiti, D., Nevola, L., Botta, B. Oxazolidin-2-one ring, a popular framework in synthetic organic chemistry: Part 2. Applications and modifications (2007) Current Organic Synthesis, 4 (3), pp. 238-307. 

36.  Botta, B., D'Acquarica, I., Delle Monache, G., Subissati, D., Uccello-Barretta, G., Mastrini, M., Nazzi, S., Speranza, M. Synthesis and host-guest studies of chiral N-linked peptidoresorc[4]arenes (2007) Journal of Organic Chemistry, 72 (24), pp. 9283-9290. 

37.  Botta, B., Delle Monache, G., Fraschetti, C., Nevola, L., Subissati, D., Speranza, M. Gaseous- versus solution-phase recognition of some aromatic amino esters by 2,8,14,20-tetrakis(L-valinamido)[4]resorcinarene (2007) International Journal of Mass Spectrometry, 267 (1-3), pp. 24-29. 

38.  Botta, B., D'Acquarica, I., Delle Monache, G., Nevola, L., Tullo, D., Ugozzoli, F., Pierini, M. Nitrosonium complexes of resorc[4]arenes: Spectral, kinetic, and theoretical studies (2007) Journal of the American Chemical Society, 129 (36), pp. 11202-11212. 

39.  Zappia, G., Menendez, P., Delle Monache, G., Misiti, D., Nevola, L., Botta, B. The contribution of oxazolidinone frame to the biological activity of pharmaceutical drugs and natural products (2007) Mini-Reviews in Medicinal Chemistry, 7 (4), pp. 389-409. 

40.  Lima, C.S.D.A., Zappia, G., Delle Monache, G., Botta, B. Uncommon 5-methoxyisoflavans from Desmodium canum (2006) European Journal of Organic Chemistry, (24), pp. 5445-5448. 

41.  Botta, B., Caporuscio, F., D'Acquarica, I., Delle Monache, G., Subissati, D., Tafi, A., Botta, M., Filippi, A., Speranza, M. Gas-phase enantioselectivity of chiral amido[4]resorcinarene receptors (2006) Chemistry - A European Journal, 12 (31), pp. 8096-8105. 

42.  Vitali, A., Pacini, L., Bordi, E., De Mori, P., Pucillo, L., Maras, B., Botta, B., Brancaccio, A., Giardina, B. Purification and characterization of an antifungal thaumatin-like protein from Cassia didymobotrya cell culture (2006) Plant Physiology and Biochemistry, 44 (10), pp. 604-610. 

43.  D'Acquarica, I., Nevola, L., Delle Monache, G., Gács-Baitz, E., Massera, C., Ugozzoli, F., Zappia, G., Botta, B. Cyanoresorc[5]arenes: Isolation, conformation and crystal structure (2006) European Journal of Organic Chemistry, (16), pp. 3652-3660. 

44.  Botta, B., Caporuscio, F., Subissati, D., Tafi, A., Botta, M., Filippi, A., Speranza, M. Flattened cone 2,8,14,20-tetrakis(L-valinamido)-[4]resorcinarene: An enantioselective allosteric receptor in the gas phase (2006) Angewandte Chemie - International Edition, 45 (17), pp. 2717-2720. 

45.  Pasqua, G., Silvestrini, A., Monacelli, B., Mulinacci, N., Menendez, P., Botta, B. Triterpenoids and ellagic acid derivatives from in vitro cultures of Camptotheca acuminata Decaisne (2006) Plant Physiology and Biochemistry, 44 (4), pp. 220-225. 

46.  Botta, B., Delle Monache, G., Menendez, P., Boffi, A. Novel prenyltransferase enzymes as a tool for flavonoid prenylation (2005) Trends in Pharmacological Sciences, 26 (12), pp. 606-608. 

47.  Botta, B., Cassani, M., D'Acquarica, I., Subissati, D., Zappia, G., Delle Monache, G. Resorcarenes: Hollow building blocks for the host-guest chemistry(2005) Current Organic Chemistry, 9 (12), pp. 1167-1202. 

48.  Botta, B., Vitali, A., Menendez, P., Misiti, D., Delle Monache, G. Prenylated flavonoids: Pharmacology and biotechnology (2005) Current Medicinal Chemistry, 12 (6), pp. 713-739. 

49.  Botta, B., Cassani, M., D'Acquarica, I., Misiti, D., Subissati, D., Delle Monache, G. Resorcarenes: Emerging class of macrocyclic receptors (2005) Current Organic Chemistry, 9 (4), pp. 337-355. 

50.  Ferrari, F., Pasqua, G., Monacelli, B., Cimino, P., Botta, B. Xanthones from calli of Hypericum perforatum subsp. Perforatum (2005) Natural Product Research, 19 (2), pp. 171-176. 

51.  Tafi, A., Botta, B., Botta, M., Delle Monache, G., Filippi, A., Speranza, M. Chiral recognition by resorcin[4]arene receptors: Intrinsic kinetics and dynamics (2004) Chemistry - A European Journal, 10 (17), pp. 4126-4135. 

52.  Botta, B., Subissati, D., Tafi, A., Delle Monache, G., Filippi, A., Speranza, M. Cavity effects on the enantioselectivity of chiral amido[4]resorcinarene stereoisomers (2004) Angewandte Chemie - International Edition, 43 (36), pp. 4767-4770. 

53.  Vitali, A., Giardina, B., Delle Monache, G., Rocca, F., Silvestrini, A., Tafi, A., Botta, B. Chalcone dimethylallyltransferase from Morus nigra cell cultures. Substrate specificity studies (2004) FEBS Letters, 557 (1-3), pp. 33-38.

 

Patents

1.  B. Botta; A. Gulino; M. Botta; M. Mori; L. Di Marcotullio; P. Infante; F. Ghirga; S. Toscano; C. Ingallina; R. Alfonsi (2014). Multitarget hedgehog pathway inhibitors and uses thereof, PCT EP 2014063449.

2.   B. Botta; A. Gulino; M. Botta; M. Mori; L. Di Marcotullio; P. Infante; F. Ghirga; S. Toscano; C. Ingallina; R. Alfonsi (2013). Multitarget hedgehog pathway inhibitors and uses thereof, RM2013A000366

3.   A. Nunziata, G. Lionetti, E. Pierri, B. Botta, G. Cancelliere, I. D’acquarica, G. Delle Monache, F. Gasparrini, L. Nevola, D. Subissati, C. Villani (2007). Method of reducing the level of nitrogen oxides in a medium by absorption with resorcin[4]arenes. EP1738821, British American Tobacco Italia S.P.A.

4.   A. Nunziata, G. Lionetti, E. Pierri, B. Botta, G. Cancelliere, I. D’acquarica, G. Delle Monache, F. Gasparrini, L. Nevola, D. Subissati, C. Villani, M. Cassani  (2006). Synthesis of resorcin[4]arenes and their use to adsorb nitrogen oxides from tobacco smoke. WO2006136950, British American Tobacco-Italia s.r.l.

5.  G. Delle Monache; F. Delle Monache; M. Carmignani; S. C. Bonnevaux; R. Espinal; C. De Luca; B. Botta (1991). Guanidine derivatives having hypotensive activity, composition containing them and process for obtaining them, PCT US 5059624 A

6.  G. Delle Monache; F. Delle Monache; B. Botta; S. C. Bonnevaux; R. Espinal; C. De Luca; M. Carmignani (1989). Guanidine derivatives having hypotensive activity, composition containing them and process for obtaining them, EP 330629 A2 19890830.

 

Selected Books

1.        Menendez, P.; D'Acquarica, I.; Delle Monache, G.; Ghirga, F.; Calcaterra, A.; Barba, M.; Macone, A.; Boffi, A.; Bonamore, A.; Botta, B.  Production of bioactives compounds: The importance of Pictet-Spengler reaction in the XXI century. In   Plant Bioactives and Drug Discovery: Principles, Practice, and Perspectives. John Wiley & Sons, Inc., Hoboken, NJ-USA, 2012.

2.        Botta, Bruno editor Chimica Organica Essenziale, 2012 Edi.Ermes s.r.l. Milano.

3.        Botta, Bruno editor Chimica Organica 2011 Edi.Ermes s.r.l. Milano.